Patch-Type Wearable Enzymatic Lactate Biofuel Cell With Carbon Cloth Bioelectrodes for Energy Harvesting From Human Sweat


Enzyme-powered biofuel cells (EBFCs) are one of the most possible power sources for wearable microelectronic devices because of their small size and specificity of enzymes. The optimum realization of these energy sources becomes possible by fabricating biocompatible, flexible, and environment-friendly electrode materials with high active sites. Nanomaterials, with enhanced surface area, fulfill the above necessities and are used in a variety of applications. In this work, a novel membraneless enzymatic biofuel cell utilizing carbon nanotubes coated carbon cloth (CC) as bioelectrodes is presented. The bioelectrodes were integrated onto an article substrate to develop an economical and disposable patch-type wearable EBFC (W-EBFC). Such W-EBFC is designed which can deliver a power density of 132 and 104μW /cm 2 , with a stable voltage in 8-mM lactate concentration and real samples of human sweat, respectively. The results reflect that CC-based bioelectrodes-based biofuel cells have enormous potential to be a good energy source for wearable biomedical applications.
Date of Publication: March 8, 2022
Electronic ISSN: 2768-167X
Publisher: IEEE
MEMS, Microfluidics and Nanoelectronics Laboratory, Birla Institute of Technology and Science Pilani (BITS Pilani) at Hyderabad, Hyderabad, India
U. S. Jayapiriya received the B.Tech. degree in electronics from the National Institute of Technology at Puducherry, Karaikal, India, in 2016, and the M.Tech. degree in nanotechnology from the National Institute of Technology Karnataka at Surathkal, Mangaluru, India, in 2019. She is currently pursuing the Ph.D. degree with the MEMS, Microfluidics and Nanoelectronics (MMNE) Laboratory, Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science Pilani (BITS Pilani) at Hyderabad, Hyderabad, India.
During her M.Tech. degree, she has worked on developing gold nanoparticles decorated aptamer-based tuberculosis detection for point of care diagnostics. She is also working under the supervision of Prof. S. Goel to develop microfluidic devices for biofuel cells for energy harvesting and sensing.
MEMS, Microfluidics and Nanoelectronics Laboratory, Birla Institute of Technology and Science Pilani (BITS Pilani) at Hyderabad, Hyderabad, India
Sanket Goel (Senior Member, IEEE) received the B.Sc. degree in H-physics from the Ramjas College, Delhi University, New Delhi, India, in 1998, the M.Sc. degree in physics from IIT Delhi, New Delhi, in 2000, and the Ph.D. degree in electrical and computer engineering from The University of Alberta, Edmonton, AB, Canada, in 2006.
He headed the Research and Development Department and worked as an Associate Professor with the University of Petroleum and Energy Studies, Dehradun, India, from 2011 to 2015. He is currently the Principal Investigator with the MEMS, Microfluidics and Nanoelectronics (MMNE) Laboratory, and a Professor with the Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science Pilani (BITS Pilani) at Hyderabad, Hyderabad, India. He has more than 250 publications, 17 patents to his credits, delivered more than 85 invited talks, and guided/guiding 40 Ph.D. and ten master’s students. His lab is focusing upon developing smart sensors and energy harvesters for diversified applications, and implementing several Indian and overseas funded projects.
Dr. Goel has won several awards, including the Best Student Paper Award in 2005, the Ph.D. Thesis Award in 2005, the Young Scientist Award in 2013, the Fulbright-Nehru Fellowship in 2015, the JSPS Fellowship in 2021, and the Best Faculty Award in 2021. He is also the Associate Editor of IEEE Sensors Journal, IEEE Transactions on Nanobioscience, Applied Nanoscience, and Journal of Nanobiotechnology. He is also in the Editorial Board of Journal of Micromechanics and Microengineering, and holds visiting appointment with UiT, The Arctic University of Norway.

1.H. Wu, Y. Huang, F. Xu, Y. Duan and Z. Yin, "Energy harvesters for wearable and stretchable electronics: From flexibility to stretchability", Adv. Mater., vol. 28, no. 45, pp. 9881-9919, Dec. 2016.

2.M. J. Cima, "Next-generation wearable electronics", Nature Biotechnol., vol. 32, no. 7, pp. 642-643, Jul. 2014.

3.S. Imani, P. P. Mercier, A. J. Bandodkar, J. Kim and J. Wang, "Wearable chemical sensors: Opportunities and challenges", Proc. IEEE Int. Symp. Circuits Syst. (ISCAS), pp. 1122-1125, May 2016.

4.P. Song, G. Yang, T. Lang and K.-T. Yong, "Nanogenerators for wearable bioelectronics and biodevices", J. Phys. D Appl. Phys., vol. 52, no. 2, Jan. 2019.

5.A. Sekretaryova, Powering Wearable Bioelectronic Devices, Amsterdam, The Netherlands:Elsevier, 2020.

6.D. Kashyap et al., "Recent developments in enzymatic biofuel cell: Towards implantable integrated micro-devices", Int. J. Nanoparticles, vol. 8, no. 1, pp. 61-81, 2015.

7.C. Gonzalez-Solino and M. Lorenzo, "Enzymatic fuel cells: Towards self-powered implantable and wearable diagnostics", Biosensors, vol. 8, no. 1, pp. 11, 2018.

8.P. Rewatkar, V. P. Hitaishi, E. Lojou and S. Goel, "Enzymatic fuel cells in a microfluidic environment: Status and opportunities. A mini review", Electrochem. Commun., vol. 107, Oct. 2019.

9.A. J. Bandodkar and J. Wang, "Wearable biofuel cells: A review", Electroanalysis, vol. 28, no. 6, pp. 1188-1200, Jun. 2016.

10.J. Kim et al., "Wearable bioelectronics: Enzyme-based body-worn electronic devices", Accounts Chem. Res., vol. 51, no. 11, pp. 2820-2828, Nov. 2018.

11.U. S. Jayapiriya, P. Rewatkar and S. Goel, "Miniaturized polymeric enzymatic biofuel cell with integrated microfluidic device and enhanced laser ablated bioelectrodes", Int. J. Hydrogen Energy, vol. 46, no. 4, pp. 3183-3192, Jan. 2021.

12.A. Ramanavicius, A. Kausaite and A. Ramanaviciene, "Biofuel cell based on direct bioelectrocatalysis", Biosensors Bioelectron., vol. 20, no. 10, pp. 1962-1967, Apr. 2005.

13.R. A. Escalona-Villalpando, E. Ortiz-Ortega, J. P. Bocanegra-Ugalde, S. D. Minteer, J. Ledesma-García and L. G. Arriaga, "Clean energy from human sweat using an enzymatic patch", J. Power Sources, vol. 412, pp. 496-504, Feb. 2019.

14.U. S. Jayapiriya, P. Rewatkar and S. Goel, " Direct electron transfer based microfluidic glucose biofuel cell with CO 2 laser ablated bioelectrodes and microchannel ", IEEE Trans. Nanobiosci., May 2021.

15.Y. Yang, X. Yang, Y. Tan and Q. Yuan, "Recent progress in flexible and wearable bio-electronics based on nanomaterials", Nano Res., vol. 10, no. 5, pp. 1560-1583, May 2017.

16.P. Rewatkar, U. S. Jayapiriya and S. Goel, "Optimized shelf-stacked paper origami-based glucose biofuel cell with immobilized enzymes and a mediator", ACS Sustain. Chem. Eng., vol. 8, no. 32, pp. 12313-12320, Aug. 2020.

17.U. S. Jayapiriya and S. Goel, " Surface modified 3D printed carbon bioelectrodes for glucose/O 2 enzymatic biofuel cell: Comparison and optimization ", Sustain. Energy Technol. Assessments, vol. 42, Dec. 2020.

18.H. Shi, G. Wen, Y. Nie, G. Zhang and H. Duan, "Flexible 3D carbon cloth as a high-performing electrode for energy storage and conversion", Nanoscale, vol. 12, no. 9, pp. 5261-5285, Mar. 2020.

19.S. Yin, Z. Jin and T. Miyake, "Wearable high-powered biofuel cells using enzyme/carbon nanotube composite fibers on textile cloth", Biosensors Bioelectron., vol. 141, Sep. 2019.

20.U. S. Jayapiriya and S. Goel, "Optimization of carbon cloth bioelectrodes for enzyme-based biofuel cell for wearable bioelectronics", Proc. IEEE 20th Int. Conf. Nanotechnol. (IEEE-NANO), pp. 150-154, Jul. 2020.

21.U. S. Jayapiriya and S. Goel, "Microfluidic non-enzymatic biofuel cell integrated with electrodeposited metallic catalysts on a paper based platform", J. Power Sources, vol. 510, Oct. 2021.

22.P. Rewatkar and S. Goel, "Paper-based membraneless co-laminar microfluidic glucose biofuel cell with MWCNT-fed Bucky paper bioelectrodes", IEEE Trans. Nanobiosci., vol. 17, no. 4, pp. 374-379, Oct. 2018.

23.S. C. Wang, K. S. Chang and C. J. Yuan, "Enhancement of electrochemical properties of screen-printed carbon electrodes by oxygen plasma treatment", Electrochimica Acta, vol. 54, no. 21, pp. 4937-4943, Aug. 2009.

24.M. L. Verma, M. Naebe, C. J. Barrow and M. Puri, "Enzyme immobilisation on amino-functionalised multi-walled carbon nanotubes: Structural and biocatalytic characterisation", PLoS ONE, vol. 8, no. 9, pp. 16-18, 2013.

25.M. H. Koo and H. H. Yoon, "Fabrication of carbon nanotubes and charge transfer complex-based electrodes for a glucose/oxygen biofuel cell", J. Nanosci. Nanotechnol., vol. 13, no. 11, pp. 7434-7438, Nov. 2013.

26.A. Niiyama, K. Murata, Y. Shigemori, A. Zebda and S. Tsujimura, "High-performance enzymatic biofuel cell based on flexible carbon cloth modified with MgO-templated porous carbon", J. Power Sources, vol. 427, pp. 49-55, Jul. 2019.

27.C. H. Kuo et al., "Biofuel cells composed by using glucose oxidase on chitosan coated carbon fiber cloth", Int. J. Electrochem. Sci., vol. 8, no. 7, pp. 9242-9255, 2013.

28.M. Bandapati, P. Rewatkar, B. Krishnamurthy and S. Goel, " Functionalized and enhanced HB pencil graphite as bioanode for glucose-O 2 biofuel cell ", IEEE Sensors J., vol. 19, no. 3, pp. 802-811, Feb. 2019.

29.M. E. Payne, A. Zamarayeva, V. I. Pister, N. A. D. Yamamoto and A. C. Arias, "Printed flexible lactate sensors: Design considerations before performing on-body measurements", Sci. Rep., vol. 9, no. 1, pp. 1-10, Dec. 2019.

30.M. Christwardana, "Combination of physico-chemical entrapment and crosslinking of low activity laccase-based biocathode on carboxylated carbon nanotube for increasing biofuel cell performance", Enzyme Microbial Technol., vol. 106, pp. 1-10, Nov. 2017.

31.A. Christenson, S. Shleev, N. Mano, A. Heller and L. Gorton, "Redox potentials of the blue copper sites of bilirubin oxidases", Biochimica Biophysica Acta Bioenergetics, vol. 1757, no. 12, pp. 1634-1641, Dec. 2006.

32.J. Lim, N. Cirigliano, J. Wang and B. Dunn, "Direct electron transfer in nanostructured sol–gel electrodes containing bilirubin oxidase", Phys. Chem. Chem. Phys., vol. 9, no. 15, pp. 1809-1814, 2007.

33.Y. Ogawa, Y. Takai, Y. Kato, H. Kai, T. Miyake and M. Nishizawa, "Stretchable biofuel cell with enzyme-modified conductive textiles", Biosensors Bioelectron., vol. 74, pp. 947-952, Dec. 2015.

34.R. Kumari, A. O. Osikoya, W. W. Anku, S. K. Shukla and P. P. Govender, "Hierarchically assembled two-dimensional hybrid nanointerfaces: A platform for bioelectronic applications", Electroanalysis, vol. 30, no. 10, pp. 2339-2348, Oct. 2018.

35.E. L. Tur-García, F. Davis, S. D. Collyer, J. L. Holmes, H. Barr and S. P. J. Higson, "Novel flexible enzyme laminate-based sensor for analysis of lactate in sweat", Sens. Actuators B Chem., vol. 242, pp. 502-510, Apr. 2017.

36.V. F. Curto et al., "Real-time sweat pH monitoring based on a wearable chemical barcode micro-fluidic platform incorporating ionic liquids", Sens. Actuators B Chem., vol. 171, pp. 1327-1334, Aug./Sep. 2012.

37.A. Koushanpour, M. Gamella and E. Katz, "A biofuel cell based on biocatalytic reactions of lactate on both anode and cathode electrodes—Extracting electrical power from human sweat", Electroanalysis, vol. 29, no. 6, pp. 1602-1611, Jun. 2017.

38.W. Jia, G. Valdés-Ramírez, A. J. Bandodkar, J. R. Windmiller and J. Wang, "Epidermal biofuel cells: Energy harvesting from human perspiration", Angew. Chem. Int. Ed., vol. 52, no. 28, pp. 7233-7236, Jul. 2013.

39.W. Jia et al., "Wearable textile biofuel cells for powering electronics", J. Mater. Chem. A, vol. 2, pp. 18184-18189, Jan. 2014.

40.I. Shitanda et al., "Paper-based lactate biofuel cell array with high power output", J. Power Sources, vol. 489, Mar. 2021.

41.Y. Yu, J. Zhai, Y. Xia and S. Dong, "Single wearable sensing energy device based on photoelectric biofuel cells for simultaneous analysis of perspiration and illuminance", Nanoscale, vol. 9, no. 33, pp. 11846-11850, 2017.

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